BIOMI

Last Updated on Tuesday, 13 March 2012 Published Date


 

I. GMO analytical laboratory

The genetically modified products are already here

The commercial production of transgenic, or in other words genetically modified organisms (GMO), especially plants has grown dynamically in the last decade and thus the products derived from these plants are already available in the stores.
The EU regulations clearly state that the traceability and the labelling of GM products should be ensured. According to the regulation, the threshold for labelling those GM events which are approved in the EU is 0.9%.

The fist GMO analytical laboratory in Hungary

Our laboratory was the first in 1999 to start with GMO analytics in Hungary.
The colleagues of the laboratory are recognised experts who regularly take part in the GMO analytical method validation rounds of the European Commission Joint Research Center (JRC), ISPRA.

According to the accepted methodology, the detection and quantification of the genetically modified DNA sequences is based on the polymerase chain reaction (PCR), where the sensitivity of the method is further increased by the use of fluorescently labelled probes (real-time PCR).
Detection and screening is targeted at the regulatory elements of the inserted genes (35S Promoter / Nos terminator).
If the sample is positive for any of the above elements, the next step is the identification of the GMO event, and following that the quantitative analysis of that GMO type which is behind the positive screening result, returning a percentile result of the GMO content.

The laboratory is usually issues accredited results within 3-5 working days.
Regarding further questions on sample size, methods, issuing results, prices, please refer to the Head of Laboratory.

Contact:

Head of Laboratory:
Dr. Klára Dallmann

Telephone:
+36 (28) 526-124

Telefax:
+36 (28) 526-190

E-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

 

II. DNA laboratory

Our services:

  • DNA sequencing
  • Fragment-analysis
  • Oligonucleotide synthesis

DNA sequencing

quickly and efficiently provides information on the nucleotide composition of a piece of DNA. This information is essential to every DNA-level work, should it be part of biotechnological, agricultural, human or pharmaceutical R+D work or diagnostic application.

Sequencing apparatus

The sequencing device uses fluorescent dyes and capillary electrophoresis in order to analyse 16 DNA samples in 2.5 hours. The sample can be either a linear PCR product or cloned into a plasmid. Universal sequencing primers are included in the price, but there is a possibility to order the synthesis of unique primers for sequencing.
The sequencing runs for about 700-800 base pairs.

The automated workflow and the experience of our workers enable to issue results in a short time - usually in 3-4 days.
The results are sent to the customers electronically.

Fragment analysis

The laboratory offers determination of fluorescently labelled DNA fragments, for example detection of SNPs, running STR and AFLP applications, or microsatellite analysis. Using several fluorescent dyes simultaneously makes it possible to process 300-400 samples daily.

Oligonucleotide synthesis

Customers receive the ordered oligonucleotides, primers in a short time, usually within 3-4 working days.

Apparatus

  • two ABI 3100 Genetic Analysers
  • one ABI 3400 DNA synthesiser

References

Our list of partners include universities, research institutes, biotechnological and pharmaceutical companies.

Contact

Head of Laboratory: Sándor Egedi
Telephone: +36 (28) 526-148
Telefax: +36 (28) 526-190
E-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

 

Downloads

Order form for sequencing

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